Lipase from Aspergillus sp. | Volume (ml) | Protein (Mg) | Total protein | Activity μmol/min | Total activity U/ml | Specific activity U/mg | Purification folds | Percentage yield |
Crude enzyme | 1000 | 0.52 | 520 | 110.14 | 110,140 | 211.81 | 1 | 100 |
Ammonium sulphate precipitation | 200 | 0.398 | 79.6 | 86.49 | 17,298 | 217.31 | 1.03 | 15.71 |
Dialysis | 80 | 0.233 | 18.64 | 79.96 | 6396.8 | 343.20 | 1.62 | 5.80 |
Gel filtration (G-100) | 40 | 0.110 | 4.56 | 53.96 | 2098.4 | 490.55 | 2.32 | 2.00 |