| Temperature | Incubation period | Enzyme activity (U/mL) of B. licheniformis (Mean ± SE of mean) | Enzyme activity (U/mL) of B. amyloliquefaciens (Mean ± SE of mean) | Enzyme activity (U/mL) of B. megaterium (Mean ± SE of mean) | Enzyme activity (U/mL) of control (Mean ± SE of mean) |
| 25˚C | 24 h | 0.029 ± 0.003 | 0.017 ± 0.003 | 0.012 ± 0.002 | 0.005 ± 0.001 |
| 48 h | 0.040 ± 0.003 | 0.024 ± 0.004 | 0.025 ± 0.002 | 0.004 ± 0.001 | |
| 72 h | 0.034 ± 0.003 | 0.016 ± 0.001 | 0.019 ± 0.003 | 0.004 ± 0.000 | |
| 30˚C | 24 h | 0.032 ± 0.001 | 0.041 ± 0.000 | 0.057 ± 0.004 | 0.009 ± 0.001 |
| 48 h | 0.057 ± 0.008 | 0.041 ± 0.002 | 0.033 ± 0.003 | 0.013 ± 0.014 | |
| 72 h | 0.030 ± 0.008 | 0.033 ± 0.001 | 0.030 ± 0.004 | 0.004 ± 0.001 | |
| 37˚C | 24 h | 0.033 ± 0.004 | 0.356 ± 0.014 | 0.023 ± 0.002 | 0.003 ± 0.000 |
| 48 h | 0.133 ± 0.006 | 0.417 ± 0.004 | 0.028 ± 0.001 | 0.003 ± 0.000 | |
| 72 h | 0.074 ± 0.001 | 0.377 ± 0.000 | 0.025 ± 0.002 | 0.002 ± 0.000 | |
| 45˚C | 24 h | 0.027 ± 0.002 | 0.031 ± 0.004 | 0.003 ± 0.001 | 0.001 ± 0.000 |
| 48 h | 0.010 ± 0.001 | 0.027 ± 0.000 | 0.005 ± 0.002 | 0.001 ± 0.000 | |
| 72 h | 0.020 ± 0.001 | 0.028 ± 0.006 | 0.007 ± 0.001 | 0.001 ± 0.000 | |
| 55˚C | 24 h | 0.037 ± 0.003 | 0.015 ± 0.001 | 0.002 ± 0.001 | 0.000 ± 0.000 |
| 48 h | 0.013 ± 0.001 | 0.009 ± 0.002 | 0.002 ± 0.000 | 0.000 ± 0.000 | |
| 72 h | 0.022 ± 0.000 | 0.025 ± 0.003 | 0.06 ± 0.000 | 0.000 ± 0.000 | |
| 65˚C | 24 h | 0.007 ± 0.000 | 0.001 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| 48 h | 0.009 ± 0.001 | 0.003 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 | |
| 72 h | 0.006 ± 0.000 | 0.001 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |