| Changed parameters | ||||
| Chrom. cond | Stationary phase | Prop of mobilephase (%/%) | Flow rate (mL/min) | Preparation method used |
| Stationary phase | C18 YMC (150 mm * 4.6 mm) 3 µm | C18 YMC (150 mm * 4.6 mm) 3 µm | C18 YMC (150 mm * 4.6 mm) 3 µm | C18 YMC (150 mm * 4.6 mm) 3 µm |
| Prop of mobilephase (%/%) | Acetonitrile/Water (60/40) | Acetonitrile/Water (80/20) | Acetonitrile/Water (80/20) | Acetonitrile/Water (80/20) |
| Flowrate (mL/min) | 1 | 1 | 0.8 | 0.8 |
| Wavelength (nm) | 265 | 265 | 265 | 265 |
| Injection volume (µL) | 10 | 10 | 10 | 10 |
| Run time (min) | 8 | 8 & 5 | 5 & 10 | 10 & 6 |
| Preparation method used | pharmaceutical industry | pharmaceutical industry | pharmaceutical industry | developed |
| Corresponding illustration | Figure 3 | Figure 4(a) & Figure 4(b) | Figure 5(a) & Figure 5(b) | Figure 6(a) & Figure 6(b) |