| Primer | Sequence (5’->3’) | Template strand | Length | Tm | GC% | Self compl. | Self 3’ compl. |
| se21- | GCTTCCAGTTGGTCCAGCAT | Plus | 20 | 60.61 | 55 | 5 | 3 |
| 22 | CGGGAGCTTGGCTATGTGTT | Minus | 20 | 60.39 | 55 | 4 | 0 |
| Product length | 131 |
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| se23- | CGTGGCATGTCTGAGCACTT | Plus | 20 | 60.95 | 55 | 4 | 3 |
| 24 | ACGCATGAAGAGGGGGAGAA | Minus | 20 | 60.91 | 55 | 4 | 0 |
| Product length | 198 |
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