| Instrument | Shimadzu HPLC | ||
| Column | YMC Triart C18 column (250 × 4.6 mm, 5 mm) | ||
| Column Temp. | 30˚C | ||
| Mobile phase | Time (min) | A: 0.1% TFA, water | B: 0.1% TFA, ACN |
| (Isocratic mode) | 0 | 5 | 95 |
|
| 15 | 5 | 95 |
| Detector | PDA (214 nm) | ||
| Flow rate | 0.8 mL/min | ||
| Injection volume | 20 mL | ||