	Mobile phase flow rate (mL/min)		Column temperature (˚C)		% solvent A at gradient start		Method conditions
	0.9	1.1	24	26	12	14	Method conditions
% Recovery
C12:0	96	101	96	99	87	109	100
C14:0	97	99	100	101	91	109	100
C22:6	103	102	100	101	88	111	100
C18:2	101	101	100	102	90	114	100
C16:0	98	93	103	96	106	99	100
C17:0	105	96	99	97	107	90	100
C18:1	100	99	106	99	101	90	100
C18:0	102	100	101	99	105	94	100
C20:0	102	100	103	102	101	99	100
C22:0	103	102	102	99	103	92	100
Resolution (linoleic-docosahexaenoic acid)
	1.75	1.86	2.15	1.91	1.89	2.15	2.02