| Mobile phase flow rate (mL/min) | Column temperature (˚C) | % solvent A at gradient start | Method conditions | |||
0.9 | 1.1 | 24 | 26 | 12 | 14 | ||
% Recovery |
|
|
|
|
|
|
|
C12:0 | 96 | 101 | 96 | 99 | 87 | 109 | 100 |
C14:0 | 97 | 99 | 100 | 101 | 91 | 109 | 100 |
C22:6 | 103 | 102 | 100 | 101 | 88 | 111 | 100 |
C18:2 | 101 | 101 | 100 | 102 | 90 | 114 | 100 |
C16:0 | 98 | 93 | 103 | 96 | 106 | 99 | 100 |
C17:0 | 105 | 96 | 99 | 97 | 107 | 90 | 100 |
C18:1 | 100 | 99 | 106 | 99 | 101 | 90 | 100 |
C18:0 | 102 | 100 | 101 | 99 | 105 | 94 | 100 |
C20:0 | 102 | 100 | 103 | 102 | 101 | 99 | 100 |
C22:0 | 103 | 102 | 102 | 99 | 103 | 92 | 100 |
Resolution (linoleic-docosahexaenoic acid) | |||||||
1.75 | 1.86 | 2.15 | 1.91 | 1.89 | 2.15 | 2.02 |