ref | Column | Detector | Advantages/drawbacks |
[5] | Hypersil Gold aQ (150 × 4.6 mm; 5 µm) | UV detector 210 nm | Reasonable run time (15 min) Standard UV detector Short Ultraviolet wavelength near or below cut-off of many solvents Only short chain fatty acids can be analyzed |
Hypersil Gold aQ (150 × 4.6 mm; 3 µm) | |||
[6] | Chromolith Performance-Si (100 × 4.6 mm) | ELSD detector | Monolithic column allows high flow rates due to low back pressure Complex mixtures of lipid classes can be separated. Not convenient for the separation of free fatty acid species |
[7] | Hypersil BDS C8 (200 × 4.6 mm; 5 µm) | Fluorescence detector | Fatty acids need to be derivatized Very high sensitivity |
[8] | RP Ascentis Express column (150 × 2.1 mm; 2.7 μm) | Triple quadrupole mass spectrometer-ESI | Molecule identity can be obtained Run time of 20 min May need UHPLC chromatographic system capable to undergo high back pressures |
[9] | Acclaim C30 column (250 × 3 mm; 3 µm) | Corona ultra RS Charged Aerosol Detector. | Poor resolution More sensitive than ELSD |