Hypersil Gold aQ (150 × 4.6 mm; 5 µm)

UV detector 210 nm

Reasonable run time (15 min)

Standard UV detector

Short Ultraviolet wavelength near or below cut-off of many solvents

Only short chain fatty acids can be analyzed

Hypersil Gold aQ (150 × 4.6 mm; 3 µm)


Chromolith Performance-Si (100 × 4.6 mm)

ELSD detector

Monolithic column allows high flow rates due to low back pressure

Complex mixtures of lipid classes can be separated. Not convenient for the separation of free fatty acid species


Hypersil BDS C8 (200 × 4.6 mm; 5 µm)

Fluorescence detector

Fatty acids need to be derivatized

Very high sensitivity


RP Ascentis Express column (150 × 2.1 mm; 2.7 μm)

Triple quadrupole mass spectrometer-ESI

Molecule identity can be obtained

Run time of 20 min

May need UHPLC chromatographic system capable to undergo high back pressures


Acclaim C30 column (250 × 3 mm; 3 µm)

Corona ultra RS Charged Aerosol Detector.

Poor resolution

More sensitive than ELSD