| Target | Melting temp (˚C) | Primer (5’ TO 3’) | Expected amplification | PCR product in cDNA (bp) |
| NRAMP | F: 72 R: 69 | F: GGGGGATTTGCAGGCAGGGG R: CCGTCGCCACACCCACTCTC | 116 | 116 |
| AT2G 16800 | F: 65 R: 69 | F: AGCATACCACCACCACCCGT R: TCCGCCATCACCACCACCTC | 116 | 116 |
| Alpha- tubulin | F: 72 R: 70 | F: GGGCGATGAGGATGAGGGCG R: GCAAGCCCATGTCCCAAAACCA | 134 | 134 |