| Primer name | | Purpose |
| β-F | ATGAACCCACCTTTCCATTTCC | Intermediate segment amplification |
| β-R | GTACGTCACTGTCTTATCG | |
| B26 | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTT | 3’RACE PCR for RrRUP2 |
| β-3’-F | CGGTGAGGAACAGTGTAC | |
| RrRUP2-F | ATGAACCCACCTTTCCATTTCC | Full-length cDNA for RrRUP2 |
| RrRUP2 | CTAATCCGAAGCTAATGACT | |
| Actin-F | CACTTAGCACCTTCCAGCAGATGT | qRT-PCR for RrRUP2 and Actin |
| Actin-R | CTACAACAGCAGACCTGAGTTCACT | |
| RrRUP2-Q-F | ACTCTCTCCACGGTCGTC | |
| RrRUP2-Q-R | CTCCGGTGGCTAGAACAGT | |
| RrRUP2-S-F | ACTAGTATGAACCCACCTTTCCATTTCC | Expression vector construction for RrRUP2 |
| RrRUP2-P-R | CACGTGCTAATCCGAAGCTAATGACTTTC |