| Cross combination | No. of Flowers Pollinated | No. of Ovaries with Ovules | No. of Ovules Cultured | No. of Hybrids Obtained | No. of Hybrids for qPCR | Chromosome Lacking Lines |
| (N. tabacum × N. tomentosiformis) × N. tabacum | 1947 | 1013 | 11644 | 380 | 103* | 7 |