Tests

Number of clones tested

Presence of both

Rpi-blb1 and Rpi-bt1

Presence

of Rpi-bt1

Absence of both Rpi-blb1 and Rpi-bt1

Gene specific primers identification

100

45

6

49

(2, 6, 7, 8, 9, 10, 11, 13, 16, 20, 21, 24, 26, 29, 31, 32, 33, 35, 38, 39, 41, 43, 55 57, 59, 64, 66, 68,

70, 73, 74, 76, 78, 79, 80, 81, 83, 85, 88, 91, 92, 95, 96, 98, 100)

(27, 28, 47, 56, 58, 65)

(1, 4, 5, 12,14, 15, 17, 18, 19,

22, 23, 25, 30, 34, 36, 37, 40,

42, 44, 45, 46, 48, 49, 51, 52, 53, 54, 61, 62, 63, 67,69, 71, 72, 75, 77, 82, 84, 86, 87, 89,

90, 93, 94, 97, 99, 101, 102, 103)

Detached leaf assay

100

39 Resistant (leaves had high resistance on both 4 and 7 DPI) and 6 moderate resistant (leaves showed some resistance on 7 DPI)

6 Moderate Resistant

(leaves showed some resistance on 7 DPI )

49 Susceptible

(Leaves were highly susceptible on both 4 and 7 DPI)