Protein constructs | ERAP1_R-His6 | ERAP1_R-IINFEKL |
Method | Vapor diffusion | Vapor diffusion |
Plate type | 24 Well hanging-drop plate | 24 Well hanging-drop plate |
Temperature (K) | 277 | 277 |
Protein concentration (mg∙ml−1) | 5 | 5 |
Buffer composition of protein solution Composition of reservoir solution Volume and ratio of drop Volume of reservoir (μl) | 10 mM Tris, pH 7.5, 10 mM NaCl 100 mM Tris, pH 8.5 and 8% PEG8000 2 μl, 1:1 800 | 10 mM Tris, pH 7.5, 10 mM NaCl 100 mM Tris, pH 8.5 and 16% PEG8000 2 μl, 1:1 800 |