Time (min)

Mobile Phase A (%)

Mobile Phase B (%)

0 - 5

20 - 25

80 - 75

5 - 10

25 - 35

75 - 65

10 - 28

35 - 50

65 - 50

28 - 29

50 - 80

50 - 20

29 - 39

80

20

39 - 40

80 - 20

20 - 80