Time (min) | Mobile Phase A (%) | Mobile Phase B (%) |
0 - 5 | 20 - 25 | 80 - 75 |
5 - 10 | 25 - 35 | 75 - 65 |
10 - 28 | 35 - 50 | 65 - 50 |
28 - 29 | 50 - 80 | 50 - 20 |
29 - 39 | 80 | 20 |
39 - 40 | 80 - 20 | 20 - 80 |