Time (min)

Mobile Phase A (%)

Mobile Phase B (%)

0 - 10

93 - 83

7 - 17

10 - 16

83 - 79

17 - 21

16 - 32

79 - 71

21 - 29

32 - 40

71 - 65

29 - 35

40 - 44

65 - 28

35 - 72

44 - 45

28 - 25

72 - 75