Polymorphism

Primer Sequence

DNA fragment Length

Restriction Enzyme

Restriction pattern (bp)

rs2736098 (G > A)

F: 5'AGGACGCGTGGACCGAGTGA-3'

R: 5'GGAACCCAGAAAGATGGTCTC-3'

324 bp

Bsp120I (PspOMI)

AA: 324,

AG: 324, 187, 137

GG: 137, 187

rs2735940 (T > C)

F: 5' ATCTTCTGCTTCCATTTCTTCTC 3'

R: 5'TCGTCTTGTAAATACTTAGGATTCC 3'

235 bp

MspI (HpaII)

TT: 235

TC: 235, 211, 24

CC: 211, 24.

MNS16A

F: 5'-AGGATTCTGATCTCTGAAGGGTG-3'

R: 5'-TCTGCCTGAGGAAGGACGTATG-3'

S: 243, 272 bp

L: 333, 302 bp

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