Components

Volume

Taq Buffer (10X)

2 µl

MgCl₂ (25 mM)

2.5 µl

dNTPs (25 mM)

0.25 µl

Forward Primer (10 µM)

1 µl

Reverse Primer (10 µM)

1 µl

DNA

1 µl

Taq Polymerase (1 unit/µl)

1 µl

Water

16.25 µl

Total

25 ml