| Reagent/concentration Volume (µL) for 1 sample |
| |
|
| Reaction X | Reaction Y |
| 5X Red Load Taq Master High Yield PCR Mix | 5 µL | 5 µL |
| Forward primers: | 0.5 µL of 20 pmol aBT1 | 0.5 µL of 20 pmol aFT5 |
|
| 0.5 µL of 20 pmol aCT2 | 0.5 µL of 20 pmol aDT6 |
|
| 0.5 µL of 20 pmol aET3 | 0.5 µL of 20 pmol mG10 |
|
| 0.5 µL of 20 pmol aDT4 | 0.5 µL of 20 pmol aBT11 |
|
| 0.5 µL of 20 pmol aAT8 | 0.5 µL of 20 pmol G12 |
|
| 0.5 µL of 20 pmol aFT9 |
|
| 20 pmol RVG9 Reverse Primer | 0.5 µL | 0.5 µL |
| PCR water | 15.5 µL | 16 µL |
| Total Aliquot of Mastermix | 24 µL | 24 µL |
| Template first round amplicon | 1 µL | 1 µL |
| Total reaction volume | 25 µL | 25µL |