No

Virulent Gene

Primer Sequences

Location within Gene

Size (bp)

Primer References

1

astA

5'TGCCATCAACACAGTATATCC3'

5'TCAGGTCGCGAGTGACGGC3'

797 ~ 817

912 ~ 894

116

[31]

2

vat

5'TCCTGGGCATAATGGTCAG3'

5'GTGTCAGAACGGAATTGT3'

(−)10 ~ (−)28

282 ~ 264

981

[21]

3

irp2

5'AAGGATTCGCTGTTACCGGAC3'

5'AACTCCTGATACAGGTGGC3'

22 ~ 42

434 ~ 416

413

[6] [21]

4

papC

5'TGATATCACGCAGTCAGTAGC3'

5'CCGGCCATATTCACATAA3'

1284 ~ 1304

1784 ~ 1767

501

[32]

5

iucD

5'ACAAAAAGTTCTATCGCTTCC3'

5'CCTGATCCAGATGATGCTC3'

239 ~ 259

952 ~ 934

714

[32]

6

tsh

5'ACTATTCTCTGCAGGAAGTC3'

5'CTTCCGATGTTCTGAACGT3'

132 ~ 151

955 ~ 937

824

[21]

7

CvaA/B cvi cvaC

5'TGGTAGAATGTGCCAGAGCAAG3'

5'GAGCTGTTTGTAGCGAAGCC3'

1076 ~ 1095

2056 ~ 2038

1181

[21]

8

fimH

5' GGATAAGCCGTGGCCGGTGG 3'

5' CTGCGGTTGTGCCGGAGAGG 3'

215 ~ 235

671 ~ 691

331

[28]