| Rare/Unclassified Taxa | Location | # of Reads/100ml | Remarks |
| Exiguobacterium sp. (Firmicutes) | Midpoint of warm spring | 1910 | Ability to survive in varied temperatures—hot springs and glaciers. permafrost. YT2 strain involved in biodegradation of polystrene [19] |
| Uliginosibacterium sp. (Proteobacteria) | Confluence of warm and cold spring | 10 | Most members isolated from freshwater sediments. Motile species involved in iron reduction, degradation of aromatic compounds, denitrification [20] |
| Vogesella sp. (Proteobacteria) | Confluence of warm and cold spring | 7 | Genus consists of approximately 5 species isolated from soil sediment, pond and spring water samples. Aerobic and chemoheterotrophic [22] |
| Dyadobacter sp. (Bacteroidetes) | Midpoint of cold spring | 5 | 12 known members including Dyadobactersoli (starch degrading), Dyadobacter jianguensis (degrades methyl red), Dyadobacter fermentans (associated with Zea mays) [23] |
| Methylobacterium sp. (Proteobacteria) | Midpoint of warm spring | 3 | Genus consists of 18 species found in aquatic systems, soil and hospital environments. Ability to use methanol, formate as a sole carbon source |
| RB25 class (Acidobacteria) | Cold spring outflow | 3 | Low abundance Acidobacteria detected in sludge treated soil [24] |
| Ellin6075 (Acidobacteria) | Cold spring outflow Confluence of warm and cold spring | 2 3 | Subdivision 4 of Acidobacteria with 2 known isolates—Chloroacidobacterium thermophilum and a Pyrimimonas sp.—thermophilic [25] |
| ABS-6 class (AD3 phylum) | Cold spring outflow | 1 | Dominant phyla found in cave sediment samples, close lineages to Chloroflexi and Armatimonadetes phyla [26] |
| CCU21 order (Acidobacteria) | Confluence of warm and cold spring | 1 | Detected and shown to be resistant to silver nanoparticles in a river sediment [27] |
| MB-A2-108 (Actinobacteria) | Cold spring outflow | 1 | Closest relative—Frankia (myracanod) isolated from deep marine sediments [28] |
| Rubrobacteraceae (Actinobacteria) | Cold spring outflow | 1 | Moderately thermophilic and have been isolated from hot springs, species tolerate high levels of ionizing radiation—Rubrobacter sp. [21] |
| OPB56 (Chlorobi) | Confluence of warm and cold spring | 1 | Recovered from Obsidian pools in Yellowstone National park. Detailed phylogeny but function unknown [29] |
| CO119 (Chloroflexi) | Midpoint of warm spring | 1 | No information found |
| DH61 order (Planctomycetes) | Confluence of warm and cold spring | 1 | No information found |
| S085 class (Chlorofexi) | Cold spring outflow | 1 | No information found |
| Candidatus Solibacter (Acidobacteria) | Confluence of warm and cold spring | 1 | Candidatus Solibacterusiaticus strain Ellin6067 has a 2 - 5 times larger genome than other Acidobacteria. Traits could provide metabolic, defensive and regulatory advantages [30] |