Microbes

DNA purification method

E. coli

S. enterica

L. mono

S. aureus

Z. bailii

−ΔCq ave

sd

−ΔCq ave

sd

−ΔCq ave

sd

−ΔCq ave

sd

−ΔCq ave

sd

GeneJET

−

0.12

−

0.30

−

0.29

−

0.12

−

0.46

Chelex-100

0.558

0.16

0.917

0.17

−2.318

0.27

−1.277

0.16

1.643

0.32

Direct-Chelex

0.105

0.11

0.445

0.13

−3.927

0.22

−0.522

0.11

0.142

0.18

EtNa-cf

−1.690

0.22

−1.408

0.48

−2.667

0.53

−1.307

0.22

3.050

1.19

EtNa-cf-Ch.

−1.612

0.44

−1.012

0.38

−2.125

0.88

−0.973

0.44

0.902

0.49

EtNa-cf-column

−0.218

0.39

0.017

0.12

−0.707

0.16

−1.348

0.39

0.992

0.44

Direct EtNa

−0.493

0.11

−1.093

0.65

−3.625

1.13

−1.458

0.11

1.248

2.11

Direct EtNa-Ch.

−0.378

0.12

−0.925

0.36

−2.990

0.90

−3.992

0.12

2.403

1.59

Direct-EtNa-C.

0.268

0.13

0.368

0.04

−0.757

0.11

−0.635

0.13

1.592

0.75