| S. No. | Target group | Forward Primer sequence (5'-3') | Reverse Primer sequence (5'-3') |
| 1 | Firmicutes | ATG TGG TTT AAT TCG AAG CA | AGC TGA CGA CAA CCA TGC AC |
| 2 | Bacteroidetes | CAT GTG GTT TAA TTC GAT GAT | AGC TGA CGA CAA CCA TGC AG |
| 3 | Lactobacillus | GAG GCA GCA GTA GGG AAT CTT CG | GC CAG TTA CTA CCT CTA TCC TTC TTC |
| 4 | Enterococcus | CCC TTA TTG TTA GTT GCC ATC ATT | ACT CGT TGT ACT TCC CT TGT |
| 5 | Clostridium | GCA CAA GCA GTG GAG T | CTT CCT CCG TTT TGT CAA |
| 6 | Bacteroides | ATA GCC TTT CGA AAG RAA GAT | CCA GTA TCA ACT GCA ATT TTA |
| 7 | Prevotella | CAC RGT AAA CGA TGG ATG CC | GGT CGG GTT GCA GAC C |
| 8 | Bifidobacterium | CGC GTC YGG TGT GAA AG | CCC CAC ATC CAG CAT CCA |