Strains, plasmids | Description | Source/Reference |
Strains |
|
|
E. coli |
|
|
DH5α | F-80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17 (rk−, mk+) phoAsupE44 -thi-1 gyrA96 relA1 | Novagen |
GM48 | thr-1, araC14, leuB6 (Am), fhuA31, lacY1, tsx-78, glnX44 (AS), galK2 (Oc), galT22, λ−, dcm-6, dam-3, thiE1 | Coli Genetic Stock Center (CGSC) |
HB101 | F- hsd-20 recA13 ara-14 proA2 lacY1 gal K2 rpsL20 (Str) xyl-5 mtl-1 supE44 | [36] |
B. anthracis |
|
|
7702 | , | [35] |
Plasmids |
|
|
pGEM T-Easy | Cloning vector | Promega |
pNF8 | pAT18Ω (PdltΩgfp-mut1) | [38] |
pWH1520 | Cloning vector | Mobitec |
pHT304-18Z | Promoterless lacZ vector | [41] |
pRP1028 | TmS allelic-exchange vector | [37] |
pSS4332 | I-SceI expression vector | [37] |