Strains, plasmids

Description

Source/Reference

Strains

E. coli

DH5α

F-80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17 (rk−, mk+) phoAsupE44 -thi-1 gyrA96 relA1

Novagen

GM48

thr-1, araC14, leuB6 (Am), fhuA31, lacY1, tsx-78, glnX44 (AS), galK2 (Oc), galT22, λ⁻, dcm-6, dam-3, thiE1

Coli Genetic Stock Center (CGSC)

HB101

F- hsd-20 recA13 ara-14 proA2 lacY1 gal K2 rpsL20 (Str) xyl-5 mtl-1 supE44

[36]

B. anthracis

7702

${\text{pXO1}}_{}^{+}$ , ${\text{pXO2}}_{}^{-}$

[35]

Plasmids

pGEM T-Easy

Cloning vector

Promega

pNF8

pAT18Ω (PdltΩgfp-mut1)

[38]

pWH1520

Cloning vector

Mobitec

pHT304-18Z

Promoterless lacZ vector

[41]

pRP1028

Tm^S allelic-exchange vector

[37]

pSS4332

I-SceI expression vector

[37]