| Target gene | Primer name | Primer sequence | Annealing T (˚C) | Product size | Reference |
| blaTEM | TEM-F | 5’-GCGGAACCCCTATTTG-3’ | 50 | 964 bp | |
| 5’-TCTAAAGTATATATGAGTAAACTTGGTCTGAC-3’ | 12 | ||||
| blaCTX-M | CTX-M-F | 5’-ATGTGCAGYACCAGTAARGTKATGGC-3’ | 60 | 593 bp | 12 |
| CTX-M-R | 5’-TGGGTRAARTARGTSACCAGAAYCAGCGG-3’ | ||||
| blaSHV | SHV-F | 5’-TTCGCCTGTGTATTATCTCCCTG-3’ | 50 | 854 bp | 12 |
| SHV-R | 5’-TTAGCGTTGCCAGTGYTCG-3’ | ||||
| blaOXA | OXA-IF | 5’-ATGAAAAACACAATACATATCAACTTCGC-3’ | 62 | 820 bp | 12 |
| 0XA-1R | 5’-GTGTGTTTAGAATGGTGATCGCATT-3’ | ||||
| Int1 | intM1_D | 5’-GAAAGGTCTGGTCATACATG-3’ | 50 | 500bp | 11 |
| intM1_U | 5’-ACGAGCGCAAGGTTTCGGT-3’' | ||||
| (GTG)5 | (GTG)5 | 5’-GTGGTGGTGGTGGTG-3’ | 50 | variable | 13 |