| Methods | Target amplicon | primers | Laboratory |
| RT-PCR (Verna et al. 2017) [14] | Gene N—287 bp | MvF 5’-ACAGGATTGCKGAGGACCTAT-3’ MvR 5’-VARGATAACCATGTACGGTGC-3’ | 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 |
| Semi Nested RT-PCR [19] | Gene P—300 bp | MVP2202 5’-KKCTCRTGGTWCCWRCAGGC-3’ MVP2480R 5’-TCTCTYCTGTGCCCTTTTTAATGG-3’ internal MVP A 5’-AGATGAGAGCTCTCTCGAGA-3’ | 1 |
| Real-time RT-PCR TaqMan [17] [18] | Gene F—192 bp | DMV Fu-F 5’-GGCACCATAATTAGCCAGGA-3’ DMV Fu-R 5’-GCCCAGATTTGTGCCTACAT-3’ probe unpublished | 2 |
| RT-PCR [12] | Gene N—173 bp | DMV-N-FP 5’-TGCCAGTACTCCAGGGAACATCCTTC-3’ DMV-N-RP 5’-TTGGGTCGTCAGTGTTGTCGGACCGTT-3’ | 3, 8 |
| Real-time RT-PCR [20] | Gene F—45 bp | DMV-F3 5’-TCGCCGCAGACAAATGC-3’ DMV-R1 5’-TCCCTGGATCCCACTTGG-3’ probe DMV-P2 -FAM-5’-CTTTAGTTGAGGTAGACGGAAT-MGBNFQ | 4 |
| RT-PCR [15] | Gene P—429 bp | DMV-C 5’-ATGTTTATGATCACAGCGGT-3’ DMV-P2 5’-ATTGGGTTGCACCACTTGTC-3’ | 5 |
| Real time RT-PCR sybrgreen [16] | Gene N—287 bp | P-1 (CDV) 5’-ACAGGATTGCTGAGGACCTAT-3’ P-2 (CDV) 5’-CAAGATAACCATGTACGGTGC-3’ | 9 |
| Nested PCR [11] | Gene H—612 bp-nested 200 bp | DMV-11F 5’-CCGAACCTGATGATCCATTT-3’ DMV-11R 5’-CGTAAATGTCCATCCCTGCT-3’ internal DMV-13F 5’-CATCATAGGGGGTGGTTTGA-3’ DMV-13R 5’-GGGGTGGTCTACTCTTGCAC-3’ | 11 |