Strain or plasmid

Relevant characteristics

Source

Escherichia coli

TransforMax EC100D pir+

F? mcrA ∆(mrr-hsdRMS-mcrBC) ϕ80dlacZ∆M15 ∆lacX74 recA1 endA1 araD139 ∆(ara, leu)7697 galU galK λ? rpsL nupG pir+(DHFR)

Epicentre

DH5α

supE44 ΔlacU169 (Φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Tiangen

Pseudomonas fluorescens

MS82

Wild-type strain

This study

MS82MT19

GGDEF/EAL::Tn5 derivative of MS82; Kmr

This study

MS82SD19

GGDEF/EAL::nptII derivative of MS82; Kmr

This study

Pf0-1

Wild-type strain

Newell et al. [17]

Plasmid

pUCP26

Expression vector for Pseudomonas; Tcr

West et al. [18]

pBR325

Cloning vector; Cmr, Tcr, Apr

Prentki et al. [19]

pBSL15

Kanamycin resistance gene cassette; Kmr

Alexeyev [20]

pGEM-T Easy

Cloning vector; Apr

Promega

pMT19E1

EZ-Tn5 carrying genomic DNA of MS82MT19 (digested by Pst I)); Kmr; 15 kb

This study

pMT19E2

EZ-Tn5 carrying genomic DNA of MS82MT19 (digested by Xba I); Kmr; 24 kb

This study

pUCP19

pUCP26 carrying the sensory box GGDEF/EAL domain gene, Tcr

This study

pMS1

pGEM-T Easy carrying 1-kb PCR product containing the SmaI site of the sensory box GGDEF/EAL domain gene; Apr

This study

pMS2

pBR325 carrying 1-kb PCR product containing the SmaI site of the sensory box GGDEF/EAL domain gene; Tcr

This study

pMS3

the nptII gene inserted into pMS2; Kmr, Apr

This study