Strain or plasmid | Relevant characteristics | Source |
Escherichia coli |
|
|
TransforMax EC100D pir+ | F? mcrA ∆(mrr-hsdRMS-mcrBC) ϕ80dlacZ∆M15 ∆lacX74 recA1 endA1 araD139 ∆(ara, leu)7697 galU galK λ? rpsL nupG pir+(DHFR) | Epicentre |
DH5α | supE44 ΔlacU169 (Φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Tiangen |
Pseudomonas fluorescens |
|
|
MS82 | Wild-type strain | This study |
MS82MT19 | GGDEF/EAL::Tn5 derivative of MS82; Kmr | This study |
MS82SD19 | GGDEF/EAL::nptII derivative of MS82; Kmr | This study |
Pf0-1 | Wild-type strain | Newell et al. [17] |
Plasmid |
|
|
pUCP26 | Expression vector for Pseudomonas; Tcr | West et al. [18] |
pBR325 | Cloning vector; Cmr, Tcr, Apr | Prentki et al. [19] |
pBSL15 | Kanamycin resistance gene cassette; Kmr | Alexeyev [20] |
pGEM-T Easy | Cloning vector; Apr | Promega |
pMT19E1 | EZ-Tn5 carrying genomic DNA of MS82MT19 (digested by Pst I)); Kmr; 15 kb | This study |
pMT19E2 | EZ-Tn5 carrying genomic DNA of MS82MT19 (digested by Xba I); Kmr; 24 kb | This study |
pUCP19 | pUCP26 carrying the sensory box GGDEF/EAL domain gene, Tcr | This study |
pMS1 | pGEM-T Easy carrying 1-kb PCR product containing the SmaI site of the sensory box GGDEF/EAL domain gene; Apr | This study |
pMS2 | pBR325 carrying 1-kb PCR product containing the SmaI site of the sensory box GGDEF/EAL domain gene; Tcr | This study |
pMS3 | the nptII gene inserted into pMS2; Kmr, Apr | This study |