Genes | Primers | Primer sequence (5’-3’) | PCR conditions | Amplicons | References |
16SrRNA | Hpx1a | CTGGAGARACTAAGYCCTCC | 94˚C, 1 min; 59˚C, 1 min; 72˚C, 1 min (40 cycles) | 150 bp | Scholte et al., 1997 |
Hpx2 | GAGGAATACTCATTGCGAAGGCGA | ||||
vacA s1/s2 | SA | ATGGAAATACAACAAACACAC | 94˚C, 45 s; 54˚C, 45 s; 72˚C, 45 s (40 cycles) | s1: 176 bp s2: 203 bp | Atherton et al., 1995 |
SCa | CCTGARACCGTTCCTACAGC | Van Doorn et al., 1998 | |||
vacA m1/m2 | MAa | CACAGCCACTTTYAATAACGA | 94˚C, 45 s; 55˚C, 45 s; 72˚C, 1 min (35 cycles) | m1: 400 bp m2: 475 bp | Van Doorn et al., 1998 |
MB | CGTCAAAATAATTCCAAGGG | ||||
cagA | Cag1 | ATGACTAACGAAACTATTGATC | 94˚C, 1 min; 53˚C, 1 min; 72˚C, 1 min (40 cycles) | 232 bp | Rasmussen et al., 2010 |
Cag2 | CAGGATTTTTGATCGCTTTATT | ||||
dupA | Dupa1 | CGTGATCAATATGGATGCTT | 94˚C, 45 s; 52˚C, 45 s; 72˚C, 45 s (35 cycles) | 197 bp | Gomes et al., 2008 |
Dupa2 | TCTTTCTAGCTTGAGCGA | ||||
oipA | HPO638F | GTTTTTGATGCATGGGATTT | 94˚C, 45 s; 57˚C, 45 s; 72˚C, 45 s (35 cycles) | 401 bp | Ben Mansour et al., (2010) |
HPO638R | GTGCATCTCTTATGGCTTTG |