The targeted gene

Primer sequence

Melting temperature (˚C)

band Size, bp

Reference

virA

F-CTGCATTCTGGCAATCTCTTCACATC

R-TGATGAGCTAACTTCGTAAGCCCTCC

58

215

[28]

ial

F-CTGGATGGTATGGTGAGG

R-GGAGGCCAACAATTATTTCC

58

320

[28]

sen

F: 5'-ATGTGCCTGCTATTATTTAT-3'

R: 5'-CATAATAATAAGCGGTCAGC-3'

60

799

[28]

set1A

F: 5'-TCACGCTACCATCAAAGA-3'

R: 5'-TATCCCCCTTTGGTGGTA-3'

55

309

[28]

set1B

F: 5'-GTGAACCTGCTGCCGATATC-3'

R: 5'-ATTTGTGGATAAAAATGACG-3'

55

147

[28]

sat

F: 5'-ACTGGCGGACTCATGCTGT-3'

R: 5'-AACCCTGTAAGAAGACTGAGC-3'

55

387

[29]

ipaBCD

F: 5'-GCTATAGCAGTGACATGG-3'

R: 5'-ACGAGTTCGAAGCACTC-3'

60

612

[30]

ipaH

F: 5'-TGGAAAAACTCAGTGCCTCT-3'

R: 5'-CCAGTCCGTAAATTCATTCT-3'

58

423

[31]

stx

F: 5'-CAGTTAATGTGGTTGCGAAG-3'

R: 5'-CTGCTAATAGTTCTGCGCATC-3'

60

895

[28]