| S. No. | Gene | Primers (5’ to 3’) | Tm (˚C) |
| 1 | Forward RRAGC | ACGGTGGTTGCATTAGTTCC | 59.9 |
| 2 | Reverse RRAGC | AAGGGGACTGTGGGAAAACT | 50.0 |
| 3 | Forward PSMC2 | AAGCAAGGAAGGTGCTGTGT | 59.9 |
| 4 | Reverse PSMC2 | AAGGCAATATCCCCCTCATC | 60.1 |
| 5 | Forward CKAP4 | TCTTCCTGACGCATCTTGTG | 60.0 |
| 6 | Reverse CKAP4 | GTCGAAACCTGTGCATTCCT | 60.1 |
| 7 | Forward MANF | CTGAGCACAGTGGACCTGAA | 60.0 |
| 8 | Reverse MANF | GGTGCAACAGAGATTGAGCA | 60.0 |
| 9 | Forward CTR9 | AGTCAGGGAGAAGCCACTCA | 59.9 |
| 10 | Reverse CTR9 | TTGTTGGATCCCTCTGGTTC | 60.0 |
| 11 | Forward CNTNAP2 | GGATGCTCTACAGCGACACA | 60.0 |
| 12 | Reverse CNTNAP2 | TCTCCATTCCAATCCAGAGG | 60.0 |