Characteristics of the culture | Medium of cultivation (RPMI-1640, FBS, L-glutamine, penicillin and streptomycin), ml | Suspension of primary lymphocyte (3 × 106 cells/ml), ml | Solution of NaN3, (concentration 448 mM ), ml | PBS, ml | Complex of biologically active substances, ml | Solution of NaCl, (0.9%), ml |
Culture of negative control (K−) Culture of positive control (K+) Experimental cultures | 12 12 12 | 0.5 0.5 0.5 | - 0.5 0.5 | 0.5 - - | - - 1.0 | 1.0 1.0 - |