Characteristics of the culture

Medium of cultivation

(RPMI-1640, FBS, L-glutamine, penicillin and streptomycin), ml

Suspension of primary lymphocyte

(3 × 106 cells/ml), ml

Solution of NaN3, (concentration 448 mM ), ml

PBS,

ml

Complex of biologically active substances, ml

Solution of NaCl, (0.9%), ml

Culture of negative control (K)

Culture of positive control (K+)

Experimental cultures

12

12

12

0.5

0.5

0.5

-

0.5

0.5

0.5

-

-

-

-

1.0

1.0

1.0

-