Parameters

% Assay*

RT*

Tailing factor*

N*

Methanol concentration

A = 60%; a = 55%, a’ = 65%

98.91 − 99.2 = 0.2

2.63 − 3.23 = −0.6

1.04 − 1.03 = 0.01

5838 − 5688 = 150

Buffer solution pH

98.86 − 99.05 = −0.19

2.49 − 3.16 = −0.67

1.04 − 1.03 = 0.01

5813 − 5791 = 22

Column temperature

98.95 − 98.70 = 0.25

2.70 − 2.33 = 0.37

1.04 − 1.04 = 0.00

5825 − 5740 = 85

Mobile Phase Flow rate

98.80 − 99.30 = −0.5

2.61 − 2.71 = −0.10

1.03 − 1.06 = − 0.03

5785 − 5775 = 10

Methanol supplier

99.07 − 97.4 = 1.67

2.66 − 2.36 = 0.3

1.04 − 1.04 = 0.00

5812 − 5773 = 39