Parameters

1

2

3

4

5

6

7

8

9

10

Methanol concentration in mobile phase

A

A

a

a’

A

A

A

A

A

A

Buffer solution pH

B

b

B

B

b’

B

B

B

B

B

Column temperature

C

C

C

C

C

c

C

C

C

c’

Mobile Phase Flow rate

D

D

D

D

D

D

d

D

d’

D

Methanol supplier

E

E

E

E

E

E

E

e

E

E