Sr. No.

Parameter

Conditions used for Analysis

Filgrastim

Etanercept

1

Stationary phase

Silica gel 60 RP-18 F254sHPTLC plates

Silica gel 60 RP-18 F254sHPTLC plates

2.

Mobile phase

Water: n-butanol (7.5:2.5 v/v)

Isopropyl alcohol: water (6.5:4.5 v/v)

3.

Detection Wavelength

225 nm

222 nm

4.

Saturation time

15 min.

10 min

5.

Band width

6 mm

6 mm

6.

Retention Factor

0.45 ± 0.07

0.32 ± 0.03

7.

Densitogram