Sr. No. | Parameter | Conditions used for Analysis | |
Filgrastim | Etanercept | ||
1 | Stationary phase | Silica gel 60 RP-18 F254sHPTLC plates | Silica gel 60 RP-18 F254sHPTLC plates |
2. | Mobile phase | Water: n-butanol (7.5:2.5 v/v) | Isopropyl alcohol: water (6.5:4.5 v/v) |
3. | Detection Wavelength | 225 nm | 222 nm |
4. | Saturation time | 15 min. | 10 min |
5. | Band width | 6 mm | 6 mm |
6. | Retention Factor | 0.45 ± 0.07 | 0.32 ± 0.03 |
7. | Densitogram |
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