Time (min)

H₂O/

0.025% FA/0.012% NH₃ (%)

ACN/

0.025% FA/0.012% NH₃ (%)

Flow (ml/min)

0

98

2

0.80

20

50

50

0.80

25

0

100

0.80

28

0

100

0.80

30

98

2

0.80