Compound	Retention time (min)	Quality control concentration (ng/mL)	Matrix effect (%)	Extraction recovery (%)	LOD (ng/mL)
(-)-Cotinine	4.6	1	97.9	102.7	0.1
		10	98.9	73.6
		100	100.2	79.4
(-)-Nicotine	4.4	1	97.0	69.1	0.2
		10	95.5	73.0
		100	100.4	70.9
(+)-Bicuculline	7.5	1	93.2	106.1	0.02
		10	95.2	101.3
		100	93.5	97.7
14-Anisoylaconine	9.7	2	83.8	81.8	0.4
		19	76.8	70.5
		189	74.5	74.5
Aconitine	11.5	1	56.0	59.8	0.2
		10	59.2	55.2
		100	59.4	51.8
Atropine	6.9	1	102.4	94.8	0.01
		10	101.2	86.7
		100	100.4	84.5
Benzoylhypaconine	9.5	1	85.0	85.3	0.5
		9	78.8	84.7
		94	76.1	88.8
Benzoylmesaconine	9.0	2	90.9	84.9	0.4
		19	83.8	88.2
		188	80.5	94.1
Cocaine	8.9	1	93.1	81.8	0.05
		10	92.5	89.4
		100	95.3	85.2
Colchicine	7.6	1	94.2	94.0	0.05
		10	94.1	85.4
		100	90.8	78.1
Ephedrine	5.4	1	102.7	72.6	0.2
		10	102.0	77.7
		100	99.9	79.6
Galantamine	5.3	1	96.3	102.1	0.02
		10	98.1	100.4
		100	98.9	99.4
Galantamine-d₆	5.3	-	-	-	-
Hypaconitine	11.6	3	56.9	68.0	0.6
		30	56.7	52.4
		300	55.6	50.4
Jesaconitine	11.6	1	58.8	57.8	0.5
		10	65.2	50.3
		100	63.5	48.5
Lycorine	4.1	1	99.4	95.6	0.02
		10	97.6	93.5
		100	99.1	90.5
Mesaconitine	11.2	2	53.1	65.2	0.4
		20	56.2	68.2
		200	55.4	69.6
Morphine	3.9	1	113.3	90.6	0.2
		10	99.3	83.4
		100	104.0	75.8
Papaverine	9.9	1	72.6	62.2	0.02
		10	79.2	53.4
		100	79.4	53.5
Pilocarpine	4.7	1	95.6	98.4	0.1
		10	98.9	89.5
		100	98.2	91.3
Protopine	9.1	1	87.8	85.1	0.2
		10	91.1	81.9
		100	91.4	85.2
Reserpine	10.6	1	72.0	40.0	0.2
		10	70.6	54.5
		100	71.5	51.3
Scopolamine	5.6	1	95.9	111.6	0.01
		10	97.8	106.6
		100	98.2	103.6
Strychnine	7.6	1	103.0	102.2	0.05
		10	101.0	98.0
		100	97.8	92.1