Concentration level (µg/mL)

Trueness

Precision

Relative bias

(%)

Repeatability

(RSD %)

Intermediate precision

(RSD %)

80.0

−0.51

0.96

0.97

90.0

−0.39

0.51

0.81

100.0

−0.32

0.60

0.71

110.0

−0.30

0.58

1.29

120.0

−0.34

0.37

0.92

Linearity: Y = 1.000X − 0.3801

R2 = 0.9964

Limit of detection (LOD) = 3.621 µg/mL

Lower limit of quantitation (LLOQ): 80.24 µg/mL

Upper limit of quantitation (ULOQ): 120.40 µg/mL