| Parameters | Trimethoprim | Sulfamethoxazole |
| Linearity | r = 0.9998 Accuracy 98.73% CV(%) 3.6 | r = 0.9996 Accuracy 98.51% CV(%) 3.9 |
| Precision and accuracy | Intra-day CV 2.95% Inter-day CV 6.1% | Intra-day CV 3.02% Inter-day CV 6.4% |
| Recovery | 92.23% | 96.85% |
| Limit of detection | 0.2 µg/ml | 3 µg/ml |
| Limit of quantification | 0.25 µg/ml | 5 µg/ml |