Parameters

CIN (100 ng∙spot⁻¹)

SD

%RSD

R_f value

Volume of mobile phase (mL)

13

0.83

0.35

0.28

15

0.53

0.38

0.30

17

0.79

0.46

0.27

Saturation time of development chamber (min)

15

4.03

0.83

0.29

20

3.97

0.82

0.30

25

5.33

1.13

0.30

Change in detection wavelength (nm)

254

4.61

0.96

0.28

258

3.60

0.74

0.30

260

5.68

1.19

0.30

Composition of mobile phase

+0.1 mL acetonitrile

0.85

0.28

0.29

−0.1 mL acetonitrile

0.62

0.31

0.28

+0.1 mL chloroform

0.58

0.44

0.31

−0.1 mL chloroform

0.55

0.39

0.29