Spiked amount

Amount detected ± SD

Mean % recovered

%RSD

% bias

50 ng (50%)

149.265 ± 0.683

99.51

1.50

0.49

100 ng (100%)

199.880 ± 1.109

99.94

1.60

0.06

150 ng (150%)

249.600 ± 0.556

99.84

1.55

0.16