Condition | Value | Recovery (%) | RSD (%) | ||
RPG | MTF | RPG | MTF | ||
Column temperature (˚C) | 23˚C | 100.24 | 101.05 | 0.31 | 0.05 |
27˚C | 100.43 | 101.12 | 0.21 | 0.02 | |
Sample temperature (˚C) | 23˚C | 100.39 | 101.00 | 0.44 | 0.10 |
27˚C | 100.12 | 101.22 | 0.42 | 0.08 | |
Mobile phase pH | 2.8 | 100.55 | 101.36 | 0.36 | 0.09 |
3.2 | 100.21 | 101.23 | 0.45 | 0.11 | |
Flow rate (mL/min) | 0.9 | 100.15 | 101.62 | 0.32 | 0.15 |
1.1 | 100.20 | 101.55 | 0.37 | 0.12 |