Tube size

Volume colloid (mL)

Volume extended semen^* (mL)

Reference

12 - 15 mL

1.0

0.25

[36]

12 - 15 mL

4.0

Up to 4.5

[32]

50 mL

15

Up to 20

[32] [33] [59] ^*

100 mL

20

25

[33]

200 mL

60

60

[32]

500 mL

150

150

[33]