Column

Enable C18 G (250 × 4.6 mm; 5 μm particle size)

Elution method

Isocratic

Mobile Phase

0.05 M KH₂PO₄ buffer (pH 4.5):Methanol:Acetonitrile (60:20:20 %v/v)

Flow rate

0.6 ml/min

Column temperature

25˚C

Volume of injection

10 µL

Detector

UV

Detection wavelength

220 nm

Run time

10 min

Diluent

Methanol (HPLC grade)