PROX 1

Figure 3(a). Mild nuclear staining of intratumoral lymphatics with PROX 1 when treated with citrate AR alone. Original magnification × 1000x.

Figure 3(b). Marked nuclear staining in the lymphatic endothelial cells in intratumoral areas with PROX 1 when treated with citrate followed by Tris-EDTA AR. Original magnification × 1000x.

Figure 3(c). Loss of cellular details in CMT for PROX 1 when subjected to citrate, followed by Tris-EDTA and pepsin treatment. Original magnification × 1000x.

VEGFR-3

Figure 4(a). Very mild immuno-staining of tumour cells in intratumoral area with VEGFR-3 when treated with citrate AR alone. Original magnification ×1000x.

Figure 4(b). Prominent cytoplasmic and mild nuclear staining in the intratumoral area with VEGFR-3 when treated with citrate followed by Tris-EDTA AR. Original magnification × 1000x.

Figure 4(c). Marked immuno-reactivity but loss of structural and cellular details of tumour cells with VEGFR-3 when subjected to citrate, followed by Tris-EDTA and pepsin treatment. Original magnification × 1000x.

VEGF-D

Figure 5(a). Slight immuno-staining of tumour cells in intratumoral area with VEGF-D when treated with citrate AR alone. Original magnification × 1000x.

Figure 5(b). Moderate granular cytoplasmic immuno-staining of neoplastic cells with VEGF-D when treated with citrate followed by Tris-EDTA AR. Original magnification × 1000x.

Figure 5(c). Marked immuno-reactivity of tumour cells but loss of structural and cellular details, with VEGF-D when subjected to citrate, followed by Tris-EDTA and pepsin treatment. Original magnification × 1000x.

Laminin

Figure 6(a). Absence of immuno-staining of blood vessels in intratumoral area with laminin when treated with citrate AR alone. Original magnification × 1000x.

Figure 6(b). Absence of immuno-staining of blood vessels in intratumoral area with laminin when treated with citrate followed by Tris-EDTA AR. Original magnification × 1000x.

Figure 6(c). Marked immuno-staining of blood vessels in intratumoral area with laminin when subjected to citrate, followed by Tris-EDTA and pepsin treatment. Original magnification × 1000x.