Different antigen retrieval methods

Citrate

Citrate followed by Tris-EDTA AR

Citrate followed by Tris-EDTA and pepsin

Podoplanin

Figure 1(a). Slight cytoplasmic immuno-reactivity for podoplanin in invading cancer cells in CMT when treated with citrate AR alone. Original magnification × 400x.

Figure 1(b). Marked immuno-staining of tumour cells for podoplanin in CMT when treated with citrate followed by Tris-EDTA AR. Original magnification × 400x.

Figure 1(c). Loss of cellular details in CMT for podoplanin when subjected to citrate, followed by Tris-EDTA and pepsin treatment. Original magnification × 400x.

LYVE-1

Figure 2(a). Slight cytoplasmic immune-reactivity for LYVE-1 in invading cancer cells when treated with citrate AR alone. Original magnification × 400x.

Figure 2(b). Marked immuno-staining of tumour cells for LYVE-1 in CMT when treated with citrate followed by Tris-EDTA AR. Original magnification × 400x.

Figure 2(c). Loss of most of the cellular details for LYVE-1 when subjected to citrate, followed by Tris-EDTA and pepsin treatment. Original magnification × 400x.