Sr. No

Name of AR solution used

Dilution used

Temp. & Time

Use

1.

EZ-AR^TM 3 is a citra based solution (10 X Concentrated)

Ten-fold dilution in deionized water (pH 6.0)

95˚C for 10 min

HIER

2.

Tris EDTA solution

Prepared by mixing Tris-6.2 g and EDTA-0.75 g in 1000 ml of distilled water (pH-9.0)

95˚C for 10 min

HIER

3.

Pepsin

2.5 mg/ml of deionized water

37˚C for 3 min

PIER