Gene

Forward primer

Reverse primer

Product Size

Annealing Temp

Insulin

CTAGTGTGCGGGGAACG

CACGCTTCTGCAGGGAC

148 bp

58

Somatostatin

ACTCCGTCAGTTTCTGCAG

CTGGGACAGATCTTCAGGTTC

136 bp

58.5

Glucagon

CATTGCTTGGCTGGTGAAAG

GCGGCAAGATTATCAAGAATGG

135 bp

58

Polypeptide

GTATGCAGCTGATCTCCGTAG

GGCATTATAAGTCCAGCGGG

149 bp

57

MafA

GAGAAGTGCCAACTCCAGAG

GCCAGCTTCTCGTATTTCTCC

101 bp

60

Pdx-1

TGAAGTCTACCAAAGCTCACG

GGAACTCCTTCTCCAGCTCTA

132 bp

58

NeuroD

CCAGGGTTATGAGACTATCACTG

TCCTGAGAACTGAGACACTCG

149 bp

60

FoxO1

GCAATGGCTATGGCAGAATG

AGTGTAACCTGCTCACTAACC

240 bp

57.5

GAPDH

AATCCCATCACCATCTTCCAG

AAATGAGCCCCAGCCTTC

122 bp

60