Samples

No inhibitor

Daniellic acid

Quercetin

Kojic acid

Concentration

50 µg/mL

100 µg/mL

50 µg/mL

20 µg/mL

V_m (mM·min⁻¹)

7.34 ± 0.21

6.89 ± 0.11

6.75 ± 0.79

4.76 ± 0.17

1.41 ± 0.102

K_m (mM)

0.28 ± 0.01

0.49 ± 0.01

0.73 ± 0.09

1.58 ± 0.11

0.29 ± 0.02

K_i (mM)

0.19 ± 0.02

0.17 ± 0.01

0.02 ± 0.001

0.03 ± 0.001

${K^{\prime }}_i$ (mM)

2.58 ± 0.72

3.67 ± 0.51

0.31 ± 0.03

0.03 ± 0.003

IC₅₀ (mM)

1.20 ± 0.06

Non determinated

0.20 ± 0.01

Inhibition type

noncompetitive mixed type

( ${K^{\prime }}_i$ > K_i)

noncompetitive pure type ( ${K^{\prime }}_i$ ~ K_i and K_m ~ IC₅₀)