Components | Initial concentration | Final concentration | Reaction volume (μL) |
Buffer taq | 10× | 0.25× | 5 |
Primer F | 20 Μm | 0.50 μM | 1 |
Primer R | 20 Μm | 0.50 μM | 1 |
d NTP | 10 Μm | 0.25× | 1 |
H2O | - | - | 39.5 |
Taq polymerase | 5 U/Μl | 0.13 | 0.5 |
ADN | - | - | 2 |
Final volume of reaction 50 μL |