Analyte

Linearity Range (pg/mL)

LODs (pg/mL)

R²

Spiked amount (pg/mL)

Precision (CV, %)

Accuracy (recovery %, mean ± SD)

DA

25 - 5000

25

0.9960

25

13.4

103.3 ± 13.9

75

6.4

95.1 ± 6.1

400

2.0

100.0 ± 2.0

4000

2.0

104.5 ± 2.0

NE

5 - 1000

5

0.9966

5

12

102.8 ± 12.4

15

10.1

88.9 ± 9.0

80

4.5

97.4 ± 4.4

800

2.6

94.1 ± 2.5

5-HT

2.5 - 500

2.5

0.9928

2.5

8.5

95.5 ± 8.1

7.5

5.8

102.6 ± 5.9

40

8.3

106.8 ± 8.9

400

5.2

101.8 ± 5.3