| System | Algae concentration (% w/v) | Reaction time (min) | Reaction temperature (˚C) | Surface potential* (mV) |
| A1 | 0.04 | 60 | 80 | −37.6 ± 1.54 |
| A2 | 0.08 | 60 | 80 | −34.6 ± 0.60 |
| A3 | 0.16 | 60 | 80 | −33.0 ± 0.36 |
| A4 | 0.24 | 60 | 80 | −42.5 ± 1.55 |