| Assay one mean Ct values with mixed targets | ||||
| Target at 6.15 × 106 plasmid copies per µL | Coxiella | A. phagocytophilum | B. burgdorferi | E. chaffeensis |
| Coxiella | 12.83 (12.35) | - | - | - |
| A. phagocytophilum | - | 13.03 (13.13) | - | - |
| B. burgdorferi | - | - | 11.69 (12.96) | - |
| E. chaffeensis | - | - | - | 12.89 (12.1) |
| Assay two mean Ct values with mixed target | ||||
| Target at 6.15 × 106 plasmid copies per µL | Rickettsia | Other non burgdorferi Borrelia | Bartonella | Babesia |
| Rickettsia | 11.13 (10.33) | - | - | - |
| Other Borrelia | - | 10.39 (10.88) | - | - |
| Bartonella | - | - | 12.08 (12.1) | - |
| Babesia | - | - | - | 24.20 (24.53) |